Vukuzazi is a population-based health ‘omics study. Designed to investigate genetic and acquired drivers of health and disease, Vukuzazi will define individual phenotypes using community-based health screening for HIV, TB and non-communicable diseases and collect biosamples to support genomic and transcriptomic study in a population of 30 000 people in the uMkhanyakude district of KwaZulu-Natal.